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PHD position Available-Protein Folding and Quality Control |
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A substantial fraction of eukaryotic genes are synthesized by ribosomes attached at the cytosolic face of the endoplasmic reticulum (ER) membrane. These polypeptides enter co-translationally in the ER lumen, which contains resident molecular chaperones and folding factors that assist their maturation. Native proteins are released from the ER lumen and are transported through the secretory pathway at their final intra- or extra-cellular destination. Folding-defective polypeptides are exported across the ER membrane into the cytosol and destroyed. Cell and organism homeostasis relies on a balanced activity of the ER folding, quality control and degradation machineries as shown by the dozens of human diseases related to defective maturation or disposal of individual polypeptides generated in the ER (e.g. cystic fibrosis, hereditary lung emphysema and neurodegenerative diseases, Hebert and Molinari Physiol. Rev. 87, 1377-1408 (2007)).
The aim of our work is to define the mechanisms that allow proper maturation of newly synthesized proteins, and the efficient and rapid disposal of terminally misfolded proteins. Experiments are performed with mammalian wild type and knockout cells in culture. Techniques used in the lab: all molecular and cell biology/biochemistry techniques such as cell culture, purification and characterization of intracellular compartments, preparation of plasmids for ectopic protein expression, radiolabeling, immunoprecipitation, immunoblotting, immunofluorescence (microscopy), chemical cross-linking, isolation and characterization of protein complexes, RNA interference, analysis of transcription and translation levels, ...
Requirements
1. Master (Diploma) in biology/cell biology/ biochemistry FROM AN EUROPEAN UNIVERSITY (Master's degree received AFTER September 2009).
2. Proficiency in English.
Applicants should send their CV to
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, shortly motivate their application (in English) and include email address of 2 referees. Selected candidates will be interviewed at the institute.
Maurizio Molinari, Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland. |
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Last Updated on Thursday, 15 April 2010 08:42 |
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Last Updated on Wednesday, 07 October 2009 09:57 |
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At the moment there are no open positions. If you would like to be notified about future positions please send an e-mail with your CV and cover letter to
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Last Updated on Wednesday, 07 October 2009 09:57 |
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At the moment there are no open positions. If you would like to be notified about future positions please send an e-mail with your CV and cover letter to
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Last Updated on Wednesday, 07 October 2009 09:58 |
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